描述：THE™ His Tag Antibody, mAb, Mouse （subtype IgG1） has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.
| 品牌 | 骋别苍厂肠谤颈辫迟/金斯瑞 | 货号 | A00186 |
|---|---|---|---|
| 规格 | 100ug | 供货周期 | 一周 |
| 应用领域 | 生物产业 |
THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞选择稳定、高产的杂交瘤细胞克隆，然后使用相应的抗原进行筛选分离，制备出特异性高、亲和力强的THE™ 系列金牌抗体。
您在实验中是否曾经因为样品量小、标签只有部分暴露、融合蛋白变性或其它棘手的问题而感到烦恼？金斯瑞THE™ 系列金牌抗体，让您的这些烦恼迎刃而解！
同时，我们也为您提供其它优质的免疫学相关产物。如凭借**的多肽合成能力，我们能够提供大多数抗体的封闭肽，让您确认观察到的信号是由抗体与靶抗原之间的相互作用产生，而不是由于非特异性结合而产生。
灵敏度—比市场上其他畅销的抗体更灵敏；
特异性—无交叉反应；
亲和力—-低解离常数碍d；
识别能力—能够识别并结合广泛的物种及表达系统的靶抗原；
低背景—无其它反应背景；
便捷—适用范围广，产物全面；
稳定—-好的批次间一致性，冻干状态下，可至少稳定保存2年。
Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
公司介绍：
欧美一区午夜福利在线是一家致力于生命科学和生物技术领域的高科技公司，依托于厦门大学人才力量。公司主要专注于细胞生物学与蛋白质分析相关产物的开发与销售。公司主要代理和自产国产优质试剂和设备，以期为生命科学多研究领域的科研人员提供美而精的产物。我们相信经过几年的努力，漠宝生物一定能成为细胞生物学、免疫学等领域优质的供应商，促进国产试剂替代进口试剂的发展，加速国产关键性原料研发进程，减轻困扰我们的进口试剂和设备依赖病。
漠宝生物将以更好的客户服务、完备的产物供应、深厚的专业知识和优秀的人脉资源，在同行业中保持良好的公司形象和竞争优势。我们将不断引进和开发优质的产物，满足广大客户的需求，在世界各国大力发展生命科学的时代背景下，我们将以为“中国生命科学发展而尽上一份绵薄之力"为使命，为科学人员创造的科研成果提供强有力的支撑。
公司将不断丰富和借助于优秀人才和技术优势，致力于研发、生产出品质优异，行业前茅的细胞生物学与蛋白质分析产物，力争在不久的将来将漠宝打造为生命科学领域的优秀品牌。
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